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Citrate Buffer pH 6.0 (10x)
Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 6 antigen retriever solution.
Preparation of Working Solution:Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
2-8°C | 64142-07 | Citrate Buffer pH 6.0 | 100 ml |
2-8°C | 64142-08 | Citrate Buffer pH 6.0 | 1000 ml |
2-8°C | 64142-08-1G | Citrate Buffer pH 6.0 | 1 Gal. |